Your command station will handle them as far as I know. Your master throttle for the system will allow you to program if it is a 402 or newer. I don't know about older ones. DecoderPro by JMRI will program them.
You need to figure out how many functions the specific throttles will handle. Then you need to figure out how many you plan to use. That will answer your specific question. The users manuals that came with the throttles will tell you that and you may be able to find copies of them on line.
My UT4R and DT402 throttles will access 12 functions. I think the 100 series may only access 4.
Remember functions are not CVs they are just used to access the various features, not program. There are multiple CVs involved in programming many of the lighting and sound features on some newer decoders. For example on the headlight, only function 0 is needed to turn it on and off but some decoders have 4-5 CVs (Control Variables) that you can program to make the headlight do different things when on or when to go off or dim etc.
