You will be near Orlando which I know has a store but I personally have never been. Colonial Photo and Hobby is the name.
If you make if south (it would be a 3 hour drive +/-) to the Miami area, Ready To Roll is my favorite spot, they built the layout in the MTH headquarters. Bill and they guys are very friendly and knowledgeable. They have 5-6 layouts and sell almost all, if not all scales.
Have a great trip!