Many discussions on this forum have touched on the subject of differences in sound between whistles, even of the same type, when mounted on the same class of locomotive.
This video on the topic of the Australian New South Wales Government Railways Standard 5-chime whistles explains it brilliantly.
Note that the air operated actuator is an on-off device. It cannot be "played" by a whistle "artist". He does not identify the manufacturer, but here in the U.S. the Viloco Air Actuator was the most common model. The Viloco system was used on Southern Pacific's GS Classes, all of New York Central's Hudsons, Mohawks and Niagaras and the N&W's J Class, although the latter also had pull ropes. Surviving audio recordings indicate that most N&W enginemen preferred to blow the whistle "old school" by using the manual method.
This explains, for instance, why the same model Hancock 3-chime sounds different on 4449, 4014, 611, 844 etc.
I personally do not care for the Viloco actuator. It ruins the sound of the beautiful New York Central six and five chime whistles, for instance. They sound much nicer whenever they surface at a "whistle blow" event and are actuated by a pull rope.