Joe, before you pul it apart try this from the EOB manual.
"Selecting the Chuff Rate EOB allows you to change the chuff rate of the locomotive, from 1, 2 or 4 chuffs per revolution while the locomotive is either stopped or running. The commands for this are as follows; ENG + ## + AUX1 + AUX1 + 5 (Each time 5 is pressed you will hear a series of whistles or horns. 1 blast for 1 chuff per rev. 2 blasts for 2 chuffs per rev. 4 blasts for 4 chuffs per rev. Pressing the sequence over and over again will simply result in the blasts repeating themselves; 1, 2, 4, 1, 2, 4, 1, 2, 4 and so on. We recommend that diesels be set to 4 chuffs per revolution (a total of 4 horn blasts). This is accomplished by pressing the AUX1 + AUX1 + 5 command three times, or until you hear 4 horn blasts."
I don't think if your chuff is generated by micro switches that you can get one chuff. Its usually a choice of 2 or 4. To get only one the switch would have to be in the two chuff position and a cam would have had to shift.
Pete