If you want to cross from a section of 3-rail track to a 2-rail section...the center roller is your enemy.
Without center rollers you won't pickup any power from the middle rail and the sprung center rollers will hit the ties on 2-rail track as the train goes around and probably derail when they come to a switch. Plus, unless you do some internal rewiring to your engines, things will short out or simply not work.
But if you want to run 3-rail trains on 2-rail track YES!!!
All of my 3-rail engines and rolling stock still use the "pizza-cutter flanges" but have been converted to Battery-Powered, Remote Control (BPRC) and running on code 148 2-rail track. The only derailments I have had were "pilot error", not throwing a switch, etc. All ,my engines have a battery and a receiver installed and are run by a handheld remote on 2.4Ghz.
Here's 2 Williams/Samhongsa engines, a N&W J 4-8-4 along with a SAL Q-3 2-8-2 sitting on Micro-Engineering code 148 track, both engines still have their original 3-rail wheels on them.
And a variety of makers rolling stock:
I don't think I've ever seen a layout that will let you simply run a 3-rail train off of 3-rail track onto 2-rail track successfully, without having to stop and turn something off, then turn something else on to continue.