Dave, you and I have similar sized layouts. I started mine back in 2003, but took 6 years off due to cancer. I've been back at it for nearly 4 years, and have a long way to go. I must admit that I do a lot of things differently from most people, which makes the whole thing take longer.
Regarding TMCC signal, I have had a lot of problems getting engines to run reliably on parts of the layout. I have two helixes. The first one was built with no special consideration for TMCC. I learned very quickly that this was a mistake, and began to retrofit it almost immediately with foil, metal window screen and wire. This helped, and most trains worked OK. Just this week I went back and put in a new wire, since the old system only went part of the way up.
When I built the second helix, I did a much better job. I applied the foil with spray adhesive directly to the bottom of each plywood segment as the construction went along. Then I connected every segment with a wire to create one continuous ground plane. Every engine works flawlessly.
My layout consists of 3 distinct levels connected by the two helixes. The lowest level is a staging/storage yard 24" above the concrete. The main level is at 42", and the upper level is 72" off the floor. If that seems a little high, I've raised the floor in the aisles 12" to help compensate. Here you can see all the levels very clearly.
The middle level was mostly built before I got sick. That's where the bulk of the signal problems are. When I started back up again, the upper deck was a blank slate, so I decided to try something different. Once the 1x4 framing was in, I put down a blanket of chicken wire, then put the plywood over it. That chicken wire is tied to earth ground via the metal electrical boxes and conduit used for the layout lighting.
Once again, having the ground plane seems to have made all the difference. Almost every engine goes around the upper deck just fine.
I'm not sure where you are in the construction process, but if you still have a chance to lay down large blankets of ground plane, I would do it! If you don't like it, you can always disconnect it from the earth ground source, but it is a lot harder to retrofit.